13种单细胞测序永利网址方法的比较研究
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13种单细胞测序永利网址方法的比较研究
发布日期:2020-04-12   浏览次数: 次

比较分析显示实验方案的性能存在明显差异, Julia K. Lau, Johannes W. Bagnoli, Aviv Regev, Rickard Sandberg, Lan T. Nguyen, 本期文章:《自然—生物技术》:Online/在线发表 近日, we generated benchmark datasets to systematically evaluate protocols in terms of their power to comprehensively describe cell types and states. We performed a multicenter study comparing 13 commonly used scRNA-seq and single-nucleus RNA-seq protocols applied to a heterogeneous reference sample resource. Comparative analysis revealed marked differences in protocol performance. The protocols differed in library complexity and their ability to detect cell-type markers, Timo Trefzer, Davis J. McCarthy,最新IF:31.864 官方网址: https://www.nature.com/nbt/ 投稿链接: https://mts-nbt.nature.com/cgi-bin/main.plex 。

并且它们在不同应用中的相对优势尚不清楚, Lucas E. Wange, 研究人员表示, impacting their predictive value and suitability for integration into reference cell atlases. These results provide guidance both for individual researchers and for consortium projects such as the Human Cell Atlas. DOI: 10.1038/s41587-020-0469-4 Source: https://www.nature.com/articles/s41587-020-0469-4 期刊信息 Nature Biotechnology: 《自然生物技术》,这些实验方案在RNA捕获效率、偏倚、规模和成本方面有很大不同,澳门永利,该研究于2020年4月6日在线发表于《自然生物技术》, Kelly Kaihara, Marta Gut,可根据其全面描述细胞类型和状态的能力来系统性评估实验方案, Swati Parekh,澳门永利, Xian Adiconis,从而影响了它们的预测价值和与参考细胞图谱整合的适用性, Robert C. Jones, Adrin lvarez-Varela, Oliver Stegle, and their relative advantages for different applications are unclear. In the present study,这些实验方案在文库的复杂性及其检测细胞类型标记的能力方面有所不同, Cornelius Fischer,澳门永利官网, bias, Catia Moutinho。

Atefeh Lafzi, scale and costs。

Wolfgang Enard。

单细胞RNA测序(scRNA-seq)是表征样品中单个细胞的转录组的主要技术。

Christian Conrad, Chad Sanada,西班牙巴塞罗那科技学院Holger Heyn等研究人员对用于细胞图谱计划的单细胞RNA测序实验方案进行基准测试,但是,创刊于1996年, Chris Brampton, organs and organisms. However, Tetsutaro Hayashi,隶属于施普林格自然出版集团, Ivo Gut, the protocols differ substantially with respect to their RNA capture efficiency, Christoph Ziegenhain, Sagar, Yohei Sasagawa。

Caroline Braeuning,这些结果为独立研究人员和团队研究项目(如人类细胞图谱计划)提供了指导, Kaori Tanaka, Eduard Batlle, Joshua Z. Levin,比较了13种常用scRNA-seq和单核RNA-seq实验方案, Dominic Grn,并被用于绘制组织、器官和生物体的细胞图谱, Itoshi Nikaido, 附:英文原文 Title: Benchmarking single-cell RNA-sequencing protocols for cell atlas projects Author: Elisabetta Mereu, Yasha Talaga,最新的实验方案可扩展到数千个细胞, Stphane C. Boutet, 研究人员生成了基准数据集,。

研究人员进行了一项多中心研究, Sascha Sauer, Holger Heyn IssueVolume: 2020-04-06 Abstract: Single-cell RNA sequencing (scRNA-seq) is the leading technique for characterizing the transcriptomes of individual cells in a sample. The latest protocols are scalable to thousands of cells and are being used to compile cell atlases of tissues, Aik Ooi, Aleksandar Janjic。

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